Pharmacy Study Material

Free pharmacy material Affinity Chromatography INTRODUCTION Affinity chromatography is highly specialised form of adsorption chromatography in which a specific ligand is immobilised chemically into an insoluble matrix to adsorb reversibly a single molecular species from a mixture of solutes. This technique is originally developed for the purification of enzymes and also extended to nucleotides. This involves the immobilisation of appropriate ligands in such a way that the enzyme is still capable of re-engaging to that immobilised form of the ligand. CLASSIFICATION OF AFFINITY CHROMATOGRAPHY This is mainly classified into two classes: Bioselective: The affinity is based on biologically relevant binding groups involving specific ligands, lectins, nucleotide cofactors, immunobiotin-labelled proteins, rarely employed cofactors, receptor proteins and immunosorbents. Chemiselective: The affinity is based on chemically defined interactions including hydrophobic chromatogr...