Pharmacy Study Material

Free pharmacy material Supercritical Fluid Chromatography INTRODUCTION Chromatography is an essential group of techniques for the separation of the compounds of mixtures by their continuous distribution between two phases i.e. stationary phase and mobile phase and the system is associated with the following: A solid stationary phase and a liquid (or) gaseous mobile phase are named as adsorption chromatography. Example: Gas chromatography (GC). A liquid stationary phase and a liquid (or) gaseous mobile phase are named as partition chromatography. Example: Paper chromatography. Advances in technology have resulted in wide range of techniques varying in complexity, separation ability, sensitivity of adsorption and partition chromatography provides an excellent separation and allows the accurate assay of very low concentrations of a wide variety of substances in complex mixtures. Supercritical fluid chromatography (SFC) is a hybrid technique of gas and liquid chr...