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Free pharmacy material Nephelometry and Turbidimetry INTRODUCTION This is mainly used to determine the scattering of the light by the suspended particles present in the sample solution. The instruments used for the measurement of the scattering are called nephelometer and turbidimeters. The choice between the nephelometry and turbidimetry depends upon the fraction of light scattered. This light scattering by the particles which are present in the colloids is known as the Tyndall affect. Nephelometry is the measurement of the scattered light by the suspended particles at right angles to the incident beam. This method is mainly used for the determination of the low concentration suspensions. Turbidimetry is the measurement of the transmitted light by the suspended particles to the incident beam. This is used for the determination of the high concentration suspensions. PRINCIPLE Light scattering is the physical character of the sample which will depend on the following:
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