Pharmacy Study Material

Free pharmacy material Mass Spectrometry INTRODUCTION Wein in 1898 proposed first crude mass spectra and identified the two neon isotopes by mass spectrometry by combining the electrostatic and magnetic fields. Aston developed mass spectra for more than 50 elements. Beynon in 1960 first wrote on the theory of mass spectrometry. Dampster proposed the instrumentation for the mass spectroscopy. Mass spectrometry is defined as the measurement and interpretation of the positive ions based on their masses. It gives the information about the molecular structure of the organic and inorganic compounds. PRINCIPLE The basic principle involved in mass spectroscopy is when the compounds are bombarded with electrons, the compound may lose one electron and forms metastable ion: M + e − M + + 2e − Again increasing the energy leads to the formation of positive ions, which is separated and recorded by the mass spectrometer based on their mass-charge ratios. Mass-charge rati...