Pharmacy Study Material

Free pharmacy material Nano Flow Liquid Chromatography INTRODUCTION The field of proteomics has experienced a fast evolution in recent years. Unlike the analysis of nucleic acids, there is not an equivalent method to polymerase chain reaction for the amplification of proteins. Thus the methodologies for proteomics have to be successfully sensitive to be able to detect endogenous levels of proteins. Due the analytical demands of biological samples, miniaturised liquid chromatography (LC) techniques were developed to allow for analysis of samples with greater sensitivity than that afforded by conventional LC, one of these methods, namely nano flow LC, with emphasis on studies that used it in combination with MS/MS. PRINCIPLE The main principle used in nano flow LC using flow rates in the range of low nano litre per minute which result in high analytical sensitivity due to the large concentrations efficiency afforded by this type of chromatography. Based on the separation, ...