Pharmacy Study Material

⚠️ Common HPLC Problems & Troubleshooting 1. High Back Pressure 🚧 Possible causes Blocked column Dirty mobile phase Blocked inline filter Precipitated buffer salts Troubleshooting Flush column with strong solvent (e.g., high % organic) Replace guard column or inline filter Filter and degas mobile phase (0.45 µm or 0.22 µm) Check tubing for blockage --- 2. Low Pressure 📉 Possible causes Leak in system Pump seal worn out Empty solvent reservoir Air in pump Troubleshooting Check for leaks in fittings Prime the pump properly Replace pump seals Ensure solvent bottle has enough mobile phase --- 3. Baseline Noise 🔊 Possible causes Air bubbles in detector Dirty flow cell Poor degassing Contaminated mobile phase Troubleshooting Degas mobile phase (sonication or helium sparging) Clean detector flow cell Purge detector Use fresh solvents --- 4. Baseline Drift 📈 Possible causes Temperature fluctuations Mobile phase composition change Column not equilibrated Detector lamp aging Troubleshoot...