Pharmacy Study Material

Free pharmacy material Size Exclusion Chromatography INTRODUCTION Size exclusion chromatography was first developed by Lathe and Ruthven in 1955. In 1959, Porath and Flodin described the separation of the macromolecules on cross-linked poly dextrane gels. J.C. Moore of Dow Company proposed the separation of the synthetic polymers on cross-linked polystyrene gels in organic mobile phases. This method is the chromatographic method in which the molecules are separated based on the size. SEC is also called as gel permeation chromatography (GEC) and gel filtration chromatography. The separation of the biomolecules in aqueous or organic mobile phase is called as the gel filtration chromatography . The separation of the organic polymers in non-aqueous mobile phase is known as GEC. PRINCIPLE The main principle involved in the SEC is diffusion through the pores present in the stationary phase with solute and solvent molecules and then removed with the continuous flow of mobile phas...