Pharmacy Study Material

Free pharmacy material Paper Chromatography INTRODUCTION The first chromatograph was invented by Mikhail Semenovich Tsvett. Again it was proposed by the Richard Martin. First, the scientists discovered that the filter paper is used as a substitute for column absorbing powder. Paper chromatography theory was first given by the Archer John porter Martin and Richard Laurence Millington Synge. Later Frederick Sanger applied this method for the determination of insulin structure. Then Melvin Calvin used this technique for the determination of energy producing cells. Watson and Crick used for determination of the DNA structure. PRINCIPLE This method is mainly used for the analysis, identification, purification and quantification of mixtures of components into individual compounds. The main principle involved is partition where the substances are distributed between the two liquids. The two liquids are the stationary phase (paper) and the moving liquid is called as the mobile pha...