Pharmacy Study Material

Free pharmacy material Spectro Fluorimetry and X-ray Fluorescence Spectroscopy INTRODUCTION Spectrofluorimetry is defined as the measurement and interpretation of the emission of the radiation after absorption. This emission of the radiation is generally called as the photoluminescence. This photoluminescence is divided into two types based on the time taken for the emission of the radiation. Fluorescence: The sample absorbs the radiant energy and emits the radiation immediately after the absorption of the radiation. The substances which show this phenomenon are called as fluorescent substances (light emitted within 10 –12 –10 –9 s). Phosphorescence: The sample absorbs the radiation and emits the radiation continuously after absorption of the radiation. The time delay of the emission of the radiation is within 10 –8 s. PRINCIPLE The main principle involved in the spectrofluorimetry and phosphorimetry is when an incident light absorbed by the sample, it undergoes ...