Pharmacy Study Material

Free pharmacy material Thin Layer Chromatography INTRODUCTION In 1949, Mein hard and Hall first proposed Thin Layer Chromatography (TLC) method by using the starch as binder to separate the inorganic ions. Kirchner proposed the conventional ascending TLC method. TLC is a solid-liquid technique in which two phases are there: one is stationary phase and other is mobile phase. The TLC principle was first introduced by the Izmailov and Shraiber. Stahl was first designed the standard equipment for the TLC. It is similar to that of the paper chromatography. PRINCIPLE The main principle involved in the TLC is the mobile phase flows through the stationary phase where a solid or liquid is supported on the solid and carries the components of mixture which will be separated into individual analytes. The separation is based upon the migration of the analytes between the stationary phase and mobile phase. The components which have more affinity towards the stationary phase elute later ...